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Spirulina samples grown in water contaminated with heavy metals have been reported to reduce protein content and impair nutritional quality

An anion exchange chromatography process for purifying a peptide or GLP-1 peptide from a mixture comprising said peptide or GLP-1 peptide and related impurities, comprising the step of: separating said peptide or GLP-1 peptide and said related impurities of said mixture by elution in a solution consisting essentially of an organic modifier, water, optionally a salt component, and optionally a buffer, with a linear or step gradient or isocratically in salt component and/or with a linear or step pH-gradient or at a constant pH-value, wherein the pH-gradient or pH-value should be in the range where said peptide or GLP-1 peptide has a negative local or overall net charge different from the local or overall negative net charge of said related impurities so as to remove said related impurities

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That matters because flushing is the uncomfortable skin redness and warmth that makes many people avoid niacin supplements entirely