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TR-FRET, high-throughput screening (HTS) and counterscreens The HTS TR-FRET is based on using lysates obtained by nondenaturing methods, thus conserving the integrity of intracellular organelles

Sirtuin 1 (SIRT1) negatively regulates GH-induced IGF-1 production in the liver by directly inhibiting signal transducer and activator of transcription 5 (STAT5) activation, which causes GH resistance under prolonged fasting, starvation, and malnutrition [6]

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-KG, also named 2-oxoglutarate, is a cofactor for 2-oxoglutarate-dependent dioxygenases (2-OGDDs), which catalyze hydroxylation reactions on diverse substrates

The bulk protein synthesis (shown by gray scale detected by western blot) of bacteria cultured with no methionine and 1,000 g/mL glutamine is significantly more abundant than that of bacteria cultured with no methionine and 10 g/mL glutamine (Figure 6K)

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