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ATP levels and basal oxygen consumption rates were reduced in the fasting group, and adenylate-activated protein kinase phosphorylation levels were decreased (64)

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This dialysis bag was then immersed in either 200 mL of phosphate-buffered saline (PBS) or a 200 mL aqueous solution of hydrogen peroxide (H 2 O 2 ) at a concentration of 10 mM, all while being maintained in a shaking culture incubator at 37 C

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