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Sensorgrams were analyzed using a global fit to the 1:1 Langmuir binding model

PANCE, which uses serial, rather than continuous, dilution, is less stringent than PACE and therefore provides evolving gene populations a greater opportunity to access a solution that survives selection before being diluted out of the lagoon 52

Statements Data availability statement The original contributions presented in the study are included in the article/Supplementary Material

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