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Once LCMS analysis was performed, Skyline software was used for metabolite identification
Thats pretty remarkable for a single compound
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After centrifugation and thorough removal of the supernatant, the pellets were incubated for 1 h in 60 l staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 l anti-BrdUFITC monoclonal antibody (BioLegend, 364104, clone 3D4)
Studies have shown that oral supplements of B12 and injections both offer effective treatment of deficiencies
For example, athletes recovering from strains or tears may experience faster healing of damaged tissues, allowing them to return to training earlier than expected
