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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST
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[7] Phospholipase C (PLC) may cleave phosphatidylinositol 4,5-bisphosphate (PIP2) into secondary messengers, IP3 and DAG (diacylglycerol)
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