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For pilot studies, method development, or any protocol that will not consume 100mg inside a sensible in-use window, the smaller vial is the technically preferable choice rather than merely the cheaper one

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Chromatographic separation was conducted on a Brownlee SPP C18 column (2.1 mm 75 mm, 2.7 m particle size, Perkin Elmer, Waltham, MA) using mobile phase A (100% H2O containing 0.1% formic acid and 1% of 1 M NH 4 OAc) and B (1:1 acetonitrile:isopropanol containing 0.1% formic acid and 1% of 1 M NH 4 OAc)

Charles was one of my patients: he had been diagnosed with B12 deficiency ten years ago