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Treatment with CDDO-Me increased NRF2 expression but not to the level observed in the liver of Gclc L-KO mice, as we could only detect substantial NRF2 expression after enriching the whole protein lysate for a nuclear fraction (Fig

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After washing three times with TBST, membranes were incubated with primary antibodies at 4 overnight

Its capacity to neutralize reactive oxygen species (ROS) helps protect nerve cells from oxidative stress, a key factor in aging and neuro-degenerative conditions (10, 43)