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After three washes with TBST, the blot was incubated with the appropriate horseradish peroxidase-conjugated secondary antibodies
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The cyclic structure increases metabolic stability further and substantially increases potency at melanocortin receptors, but the truncation and cyclization also eliminate receptor subtype selectivity
* Statistically significant
[30] The free radicals are due to the action of UV light on AGE (advanced glycation end-products) as a result of the reaction of DHA with the skin, and the intermediates, such as Amadori products (a type of AGE), that lead to them
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