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Both the Tet-inducible vector pRMC2 (Addgene, plasmid 68940) 70 and the mKate insert were digested with KpnI-HF and EcoRI-HF (New England Biolabs), gel purified using a QIAquick PCR purification kit (Qiagen), and ligated using T4 DNA ligase (New England Biolabs) at 16 C for 16 h
Because oral B12 supplements face a hostile digestive tract where up to 90% of the nutrient is lost, our clinical-grade intramuscular injections bypass the gut entirely
Solution: Use at least 250mcg per injection
"Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens." Proc Natl Acad Sci
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People call it the master antioxidant because it helps detoxify and heal cells
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