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Bile acid analysis Gallbladder bile, liver tissue homogenates, or cecal content were diluted with internal standard, vortexed, and purified by the sedimentation plate

Discard any remaining solution after that, even if the vial still has liquid in it

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Gut peptide hormones such as GLP-1 could potentially also have an impact on appetite regulation by effects on gastric emptying and direct CNS effects, but we needed a robust methodology that could be used to measure acute effect of putative hormones on subjective appetite and spontaneous food intake

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