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The reaction was halted using a 2 N H 2 SO 4 solution, and the absorbance was measured at 450 nm using a CLARIOstar microplate reader (CLARIOstar, BMG LABTECH, Germany)

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Western blot analysis Total liver protein lysates were separates on 10% and 12% SDSpolyacrylamide gel and transferred on nitrocellulose membrane

In addition, melatonin and vitamin C restored spermatogenesis by increasing the total antioxidant capacity (TAC), and decreasing nitric oxide (NO) and testicular MDA levels restored the antioxidant status

The cDNA was amplified using a Syber Green I PCR-Master Kit from Fermentas (PA, United States)

Den typiske startvariant er BPC 157 5 mg , som normalt ligger omkring ca