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GFP induction was verified at excitation and emission wavelengths of 475 nm and 508 nm, respectively, using a Synergy H1 microplate reader (BioTek)

while the T3 RNAP variant has high selectivity for the T3 promoter, it remains effectively inhibited by T7 lysozyme 23

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For the TFC assay, 500 L of 1 mg/mL sample, 100 L of 10% aluminum chloride, 100 L of 1 M potassium acetate, 1.5 mL of MeOH, and 2.8 mL of distilled water were mixed, vortexed, and allowed to react in the dark for 40 min

In regenerative medicine and wellness care, peptides are often explored for their potential ability to support the bodys natural healing and recovery processes

J Clin Invest 1984