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The cells were then harvested by trypsinization, and the level of ferrous iron was determined by imaging using a confocal microscope or by flow cytometry analysis

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Yes, the copper(II) ion is critical for GHK-Cu's biological activity

established three combinations comprising two transcription factors: HNF4 with FOXA1, FOXA2 or FOXA3, which successfully converted mouse embryonic fibroblasts (MEFs) into induced hepatocyte-like cells (iHeps) (Sekiya and Suzuki, 2011)