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Rat tissue sections were pretreated for 15 min with HIER at 95 C in ER2 (Leica) followed by a 15-min protease treatment and then hybridized with rat-specific probes to detect mRNA transcripts for CALCR (477798), PRLH (1301288-C2) and GLP1R (315228-C3
Mehrpooya, M., Rabiee, S., Larki-Harchegani, A., Fallahian, M., Moradi, A., Ataei, S., & Javad, M
At maintenance doses of 8-12mg, published protocols consume roughly one 10mg vial per week
[18] Rayman MP
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Three separate experiments were performed, once with 23 mice per group due to logistical issues, and twice with 5 mice, for a total of 1213 mice per treatment group as follows: vehicle (PBS) n = 12, L-carnitine alone n = 13, colistin alone n = 13, colistin and L-carnitine co-injection = n = 12
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