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The contribution of various families of enzymes such as conjugation catalyzing transferase, hydrolases, peroxidases, reductases, dehydrogenases, and monooxygenases results in protection against different hazardous components like N-nitroso compounds, which are very different in their cellular defense mechanism
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Gene silencing was verified by detecting proteins via immunoblotting analysis after transient transfection with siRNA
Cells were plated in rows BG and columns 210, with 100l DMEM or Hanks Balanced Salt Solution (HBSS) plated in remaining wells
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