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Protein band intensities were quantified using ImageJ software (NIH, USA) after background subtraction and normalized to -actin (for total and cytoplasmic proteins) or Histone H3 (for nuclear proteins)
Isley WL, Underwood LE, Clemmons DR
The cells also were assayed for the cytotoxicity effects of menadione using the CellTox Green Cytotoxicity Assay
erinaceus has also been shown to improve anxiety-depressive disorders in robust studies such as clinical trials ( Quercetin, a flavonoid with antioxidant properties, can potentially reduce TNF- levels and increase IL-10 levels in C
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