Using the same FRET-based assay, we compared the activity of X(PC) to that of wild-type BoNT/X protease on its native substrate VAMP1 ( K m = 6.93 M, k cat = 3.52 s 1 , k cat / K m = 0.508 s 1 M 1
In this condition of viral infection, oxidative stress is imposed by GSH depletion, occurring in two steps and following direct virus challenge of the cell membrane, without the intervention of ROS (Palamara et al., 1996)
2014 Dec;101:2-30
Role of microtubule-associated proteins in the control of microtubule assembly
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If the benefits of continued therapy outweigh the risks, three options exist: premedication (acetaminophen, diphenhydramine, and/or corticosteroids), desensitization, or use of a different therapeutic agent