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Archived from the original on 30 November 2023
M.Ghavimi-AlaghaD.StarrS
The protein was transferred to 0.2 m pore-sized nitrocellulose (wet transfer, 1 hour, 100 mA, RT) and then blocked with 5% non-fat dry milk in TBS-T (20 mmol/L TrisHCl, 150 mmol/L NaCl, 0.05% Tween 20, adjusted to pH 7.4) and incubated with the following primary monoclonal antibodies: anti--actin, anti-cleaved-PARP, anti-cleaved Caspase-3, anti-cleaved Caspase-9, and anti-COX IV (for apoptosis evaluation) or anti-LC3A/B, anti-Beclin-1, anti-Atg12, and anti-Atg-7 (for autophagy evaluation) antibodies, diluted 1:1,000 in blocking buffer overnight at 4C
doi:10.1016/j.mam.2008.08.003 Coppersmith V, Hudgins S, Stoltzfus J, et al
Keep your supplements in a cool, dark place (or the refrigerator if the label specifies) to maintain potency
Engineering such networks requires precise modification of multiple genetic components, including structural genes, transcription factors, regulatory elements, and signaling regulators (Zhang et al., 2009)
