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Her team recruited 45 participants who had each lost at least 15% of their body weight (roughly 40 pounds on average) using tirzepatide (sold as Mounjaro and Zepbound), but were stopping the drug

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RT-qPCR was performed in a 7500 fast real-time PCR system (Thermo Fisher Scientific) using TaqMan fast universal PCR master mix (Thermo Fisher Scientific) and a mixture of predesigned TaqMan primers and probes (Thermo Fisher Scientific or Integrated DNA Technologies, Coralville, IA, USA) (see Supplementary Table S1)