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Oxidized roGFP2 WT , PfGrx C88S , and PfGrx C32S/C88S were produced by incubation of the reduced enzymes with 5 mM Diamide for 60 min on ice
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However, in the present construct, GAP1 expression being under the control of pGSH1 is not subject to the repressive effect of nitrogen on pGAP1 , enabling the use of ammonium sulphate in the cultures
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References Martin, B., Lopez de Maturana, R., Brenneman, R., Walent, T., Mattson, M
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