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To qualify for weight-management treatment, patients typically need a BMI of 27 or higher, with most programs also considering weight-related health conditions such as high blood pressure, type 2 diabetes, or high cholesterol

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The Cell Ranger pipeline (10x Genomics version 3.1.0) was used to perform sample demultiplexing and to generate FASTQ files for read 1, read 2, and the i7 sample index for the gene expression and cell surface protein libraries