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Subsequently, the sperms smeared on poly-lysine-treated glass slides were fixed, permeabilised, immunolabelled with Pns11-rhodamine (0.5 g/L), and then stained with DAPI (2.0 g/mL)

7a, b, Cys 2 starvation as well as BSO and erastin treatment increased the percentage of cells in the G0/G1 phase with a concomitant decrease in the G2 phases, suggesting that GSH depletion induces growth arrest in RPE cells