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Goat antirabbit IgGbiotin secondary antibody was used as indicated by the manufacturer (Santa Cruz, sc2040)

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At varying time points (0, 2, 5, 15, 30 min), purified Pt GSTF8 (8 M) and GSH (20 M) were added to an aliquot of the equilibration reaction and the resulting mixtures were incubated at 30 C for 30 min at 1200 rpm using a 3 mm shaking diameter before extraction with 2 vol of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid

Given the close association between glutathione metabolism, the tumor microenvironment, and immune function, this form of metabolic reprogramming may substantially influence treatment responses in lung adenocarcinoma

Fresh kidney beans boiled for 10 min and dried red beans boiled for 30 min without soaking lost 14% and 19% of folate, resp