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Despite the concentrated research on mammals, the current sleep definition lacks an all-inclusive explanation to cover its evolutionary-conserved role, lending support to the hypothesis that sleep serves a fundamental need, and the best way to define sleep would be to identify this need
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Differential requirement of the extracellular domain in activation of class B G protein-coupled receptors
Results The enzyme samples are incubated in a phosphate buffer containing specific concentrations of glutathione (GSH) and peroxide and then incubated for a suitable time to react before Ellman's reagent (5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) is added
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