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Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)

10.1097/SLA.0000000000004369 3 ArnettD

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A study of the effects of hypoglycemic agents on vascular complications in patients with adult-onset diabetes

Impaired heme production, leading to blood cells with less oxygen-carrying hemoglobin and, over time, microcytic anemia

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