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In vitro experiments To plate cells for infection, aliquots of BMDMs were thawed on ice, diluted into 9 ml of DMEM, centrifuged in an Eppendorf 5810 R at 1,200 RPM (290 x G) for 5 min, and the pellet was resuspended in 10 ml BMDM media without antibiotics
During this procedure, a tube-like tool called an arthroscope is put into the joint through a small cut to see or fix an injury
Many commercial products have been approved for scaffold-associated chondrocyte implantation for more than a decade in Europe and Australia, such as Chondro-Gide (a bilayer collagen type 1/3 scaffold, manufactured by Geistlich Biomaterials, Wolhusen, Switzerland), Hyaff-11 (a hyaluronan-based scaffold, manufactured by Fida Advanced Biopolymers, Abano Terme, Italy), and BioSeed-C (a synthetic polymer scaffold composed of fibrin, polyglycolic/polylactic acid, and polydioxanone, manufactured by BioTissue, Zrich, Switzerland)
claimed that neither testosterone nor DHT was the major factor to physiologically down-regulate AR in corpora cavernosa 19,57
doi: 10.3389/fphar.2015.00098