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Proteins were removed by centrifugation at 12,000 rpm at 4 C for 20 min and the supernatant was transferred to a glass vial and sealed tightly

Shah SA, Khan M, Jo MH et al (2017) Melatonin stimulates the SIRT1/Nrf2 signaling pathway counteracting lipopolysaccharide (LPS)-induced oxidative stress to rescue postnatal rat brain