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UV exposure note: MT-II stimulates melanin production at the cellular level, but UV radiation triggers additional melanin distribution and darkening in the skin

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After filtration, centrifugation, precipitation, and resuspension, FLS were cultured in complete culture media (DMEM with 10% fetal bovine serum, 1% antibiotics) and maintained at 37 C in a humidified incubator of 5% CO 2 and 95% air