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10.1172/jci157011 157 TrachtenbergF.VichinskyE.HainesD.PakbazZ.MednickL.SobotaA.et al (2011)

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After pre-conditioning in the presence or absence of 50 nM Exendin-4 for additional 18 hours, culture medium was changed to serum-free DMEM containing 1 mM sodium pyruvate, without glucose and sodium bicarbonate