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Barenholz, Y
The supernatant, containing mitochondria, was collected and further centrifuged at the same speed to pellet any remaining heavy contaminants
You can alternate between Song mode, to hear everything, or Pattern mode, to focus on and develop individual patterns
The sequence of free roGFP2-iL and GRX1-roGFP2-iL were cloned into the BamHI and NcoI restriction sites of the protein expression vector pQE-30 (Qiagen, www.qiagen.com) for the production of a recombinant protein containing an N-terminal His 6 -tag
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