The use of humanized mice was required, as it is well-established that human albumin binds poorly to mouse FcRn 13,70,71,72,73
The Core Formula Volume to inject (mL) = Desired dose (mg) / Concentration (mg/mL) | Vial size | BWI added | Concentration | 1.25 mg dose | 1.75 mg dose | |-----------|-----------|---------------|--------------|--------------| | 10 mg | 2.0 mL | 5 mg/mL | 0.25 mL (25 units) | 0.35 mL (35 units) | | 10 mg | 1.0 mL | 10 mg/mL | 0.125 mL (12.5 units) | 0.175 mL (17.5 units) | | 5 mg | 1.0 mL | 5 mg/mL | 0.25 mL (25 units) | 0.35 mL (35 units) | | 5 mg | 2.0 mL | 2.5 mg/mL | 0.50 mL (50 units) | 0.70 mL (70 units) | On a U-100 insulin syringe, each unit mark = 0.01 mL
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Proper handling protects the delicate peptide structure and keeps the solution sterile
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