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The isolated mRNA was used to generate dual-indexed cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs)

Initially, some patients might need weekly injections, while others might benefit from monthly maintenance dosages

However, synthetic B12 in supplements is already in free form and does not require the same initial digestive processes

The compact and lightweight format also supports efficient handling, storage, and repeated evaluation during extended research projects

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5-Amino-1MQ has the formula CHN (molecular weight 159.21 g/mol)