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Glimepiride (CAROLINA) study, the cardiovascular safety of linagliptin was compared with the sulfonylurea glimepiride on top of standard of care in 6,033 subjects with type 2 diabetes and increased cardiovascular risk or established cardiovascular disease over a median follow-up period of 6 years

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A peptide should not be used to bypass a proper evaluation

The sequences of specific primers used for RT-qPCR in this study are listed in Supplementary Table S1

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Then, acetonitrile was added at 85 C until the components were dissolved completely