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Possible biases caused by the randomization process, deviations from intended interventions, missing data, outcome measurements, and selective reporting were rated as high risk, low risk, or some concerns

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For immunofluorescence, the primary antibodies used were monoclonal [8G9] to GLP-1, monoclonal SST antibody (#MAB2358), and monoclonal Human/Mouse Ghrelin Antibody (R&D systems #MAB8200), and the secondary antibodies were Alexa Fluor 488 AffiniPure Donkey Anti-Rat IgG and Cyanine Cy3 AffiniPure Donkey Anti-Mouse IgG from Jackson ImmunoResearch (Europe Ltd.) and were used at a final dilution of 1/300

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And without the continued downward trajectory that motivated continued use, exploring alternatives with different mechanisms becomes an appealing strategy to break through stalled progress

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