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To model Leydig cell senescence in vitro, TM3 mouse Leydig cells were treated with hydrogen peroxide (H2O2)
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It functions as a post-translational modulator, participating in gene transcription, apoptosis, cell cycle regulation, metabolism, and development
Both cell lines were maintained under standard conditions in specific DMEM/F-12 media supplemented with serum
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Mitochondria is a cellular organelle often referred to the "powerhouse" of the cell, regulating a range of functions from energy generation, metabolism and cell survival
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