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These proteins play significant roles in biological quality control, programmed cell death, hemostasis and catabolic processes, protein folding, cellular oxidant detoxification, coagulation, and retinal homeostasis

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In addition to traditional animal sources, recombinant DNA technology has emerged as an innovative approach, utilizing bacteria, yeast, insects, or mammalian cells as hosts for collagen production [30, 31]

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However, one of the primary drawbacks of OOAC models is the limited number of cells that can be cultured within these small devices that often are too minute for traditional biochemical detection methods such as Western blotting, gel electrophoresis, and colorimetric detection [225]

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