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During the trial, additional anti-diabetic medication was initiated as an add on to randomized treatment by 1.9% and 1.2% of patients randomized to semaglutide tablets 14 mg and empagliflozin 25 mg, respectively

This depends on the severity of symptoms and clinical assessment

The 12mg dose at 120 units (1.20mL) is borderline and can be handled with a single draw using a syringe rated for at least 1.2mL, or a clean 60/60 unit split using two standard syringes

We recommend performing spike-and-recovery experiments to assess matrix effects before analyzing new sample types

The key is managing expectations: these are supportive tools, not pharmaceutical replacements

Int J Nutrol