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Extracted proteins were quantified using a Pierce BCA Protein Assay kit

Front Aging Neurosci 10:106 Frantseva MV, Velazquez JL, Hwang PA, Carlen PL (2000) Free radical production correlates with cell death in an in vitro model of epilepsy

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Start Your MOTS-c Protocol With a Proper Assessment The MOTS-c dosage protocol requires just 4 injections over 20 days

Lysis buffer was added to the cell pellet until the volume reached 130 L, followed by thorough mixing and vortexing on ice