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Mass spectrometry analysis of metabolitessample preparation The liver, brain, kidney, and lung tissues were homogenized with a pre-chilled BioPulverizer (59012MS, BioSpec) and then placed on dry ice
Over time, both peptides have appeared in numerous scientific publications examining peptide signalling dynamics, molecular interaction pathways and regulatory communication systems
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It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile
