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Although IGFBP-3 can act as a tumor suppressor, its expression in breast cancer is highest in basal-like TNBC and correlates with shorter recurrence-free patient survival [17]

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The identity of each protein was also confirmed by probing with antibodies specific for IL-1 sRII and Lyn respectively, but due to the strength of the streptavidin-HRP signal, this was done on separate membranes where more protein was loaded per well for detection with specific antibodies

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