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doi: 10.1016/j.redox.2019.101179 29 JungMKasamatsuSMatsunagaTAkashiSOnoKNishimuraAet al
Gln is metabolized by GLS to produce alpha-ketoglutaric acid (-KG), which serves as a crucial cofactor for TET enzymes that catalyze DNA demethylation, as well as for histone demethylases like JHDM
Discard the medium and wash the cells three times with pre-cooled PBS
Do not shake or wave the syringe to clear it, that introduces air
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