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Two types of DNA polymerases were utilised in this study: KOD hot start DNA polymerase from NovgenR (Darmstadt, Germany), used to construct the SAT1 flipper disruption cassette, and TaKaRa Ex TaqTM from Takara Bio Inc
Plants have evolved enzymatic and non-enzymatic antioxidants to neutralize excess ROS
Debatri Datta September 4, 2023 Written By Dr
The procedure is done under medical supervision
Leaf EL was estimated by using the method of 41 and the following formula: Measurement of non-enzymatic antioxidants Maize leaves (0.5 g) were homogenized in 3 mL ice-cold 5% meta-phosphoric acid containing 1 mM ethylenediaminetetraacetic acid (EDTA) using a mortar and pestle and centrifuged at 11,500 g for 12 min at 4 C
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