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Coronary Heart Disease (CHD)-specific Legumes may benefit cardiovascular health by decreasing serum cholesterol concentration and by improving blood glucose and the insulin response
This mechanism operates independently from calorie restriction, meaning blood pressure improvements begin before significant weight loss occurs
After washing in PBS, horseradish peroxidase-conjugated goat anti-rabbit IgG (ab175470, Abcam) diluted 1:200 in 1% rabbit serum in PBS was applied, and the slides were incubated for 30 min at room temperature
Ultrathin, 6070 nm thick sections were cut with Leica UCT ultramicrotome (Leica Microsystems, Germany)
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