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After cryoprotection, the brainstem was separated and cut on a cryostat (Leica 1800) into 18 m coronal sections, which were mounted on microscope slides (SuperFrost Ultra Plus GOLD), for later imaging using fluorescence microscopy

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Further, this synthetic fragment increased the repressed expression of beta-3 adrenergic receptors in obese mice to levels comparable to those found in lean mice