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The assay buffer consisted of DMEM without phenol red (Gibco #11880-028) supplemented with 10 mM HEPES (Gibco #15630-056), 1X GlutaMAX (Gibco #35020-038), and 1% (w/v) ovalbumin (Sigma A5503), 0.1% (v/v) Pluronic F-68 (Gibco, #24040-032)

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However, NADP + and NADPH levels in frozen blood differ from fresh blood due to NADPH oxidation during the freeze-thaw cycle

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Figure 2 3.3.2 Possible mechanisms The relationship between H

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