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PC cells (1-5 10 6 /ml) were incubated with a probe at 37C for 20 min in a 35 mm porous glass bottom dish (Cellvis, USA) and the nuclei were stained using Hoechst (Beyotime)
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Research shows that overexpression of PER1 sensitizes human cancer cells to the effects of DNA damage-induced apoptosis
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Those following a vegan or strict vegetarian lifestyle are at greater risk of deficiency and may benefit from regular B12 supplementation or injections
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