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Detection of cell death For cell death assays, cells were washed twice in phosphate-buffered saline and then stained with Annexin V-FITC (Biovision, Mountainview, CA) and propidium iodide (PI, Sigma-Aldrich) according to the manufacturer's instructions
Global ATP receptor downregulation seems an unlikely explanation for this finding because in control experiments multiple applications of ATP induced repeated neuronal Ca 2+ responses of similar amplitudes
IL-33 induces antigen-specific IL-5 + T cells and promotes allergic-induced airway inflammation independent of IL-4
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