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Complex purification Cell pellet was thawed in 20 mM HEPES pH 7.4, 50 mM NaCl, 5 mM CaCls, 2 mM MgCl 2 supplemented with cOmplete Protease Inhibitor Cocktail tablets (Roche) and benzonase (Merk Millipore)
Specifically, the risk ratios for nausea ranged from 2.07 to 3.51 across different doses of tirzepatide, and from 2.45 to 2.84 for semaglutide (ESM Table 14)
This finding is consistent with other study that have found that amplifying the 5 UTR overestimates the frequency of subtype 1b by 20% at the expense of subtype 1a [21]
