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When encountering elevated intracellular GSH levels, these metal-based complexes break down, triggering the targeted release of photosensitizers

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Doses per vial formula Number of doses = Total vial volume in ml x Concentration in mg per ml / Weekly dose in mg Or expressed differently: Total mg in vial / Weekly dose in mg = Number of weekly doses Practical examples 5 mg/ml, 1 ml vial (5 mg total): At 0.25 mg/week: 5 / 0.25 = 20 doses (20 weeks supply) At 0.5 mg/week: 5 / 0.5 = 10 doses (10 weeks supply) At 1.0 mg/week: 5 / 1.0 = 5 doses (5 weeks supply) At 2.4 mg/week: 5 / 2.4 = ~2.1 doses (roughly 2 weeks supply) 5 mg/ml, 2 ml vial (10 mg total): At 0.25 mg/week: 10 / 0.25 = 40 doses (40 weeks supply) At 1.0 mg/week: 10 / 1.0 = 10 doses (10 weeks supply) At 2.4 mg/week: 10 / 2.4 = ~4.2 doses (roughly 4 weeks supply) 2.5 mg/ml, 1 ml vial (2.5 mg total): At 0.25 mg/week: 2.5 / 0.25 = 10 doses At 0.5 mg/week: 2.5 / 0.5 = 5 doses At 1.0 mg/week: 2.5 / 1.0 = 2.5 doses Keep in mind that syringe dead space (the medication trapped in the needle hub) reduces your effective yield by approximately 3 to 7 units per injection

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The QIAquick Gel Extraction kit was supplied by Qiagen (Valencia, CA, USA) and the Alkali-cation yeast transformation kit was obtained from Qbiogene (Heidelberg, Germany)